IgG anti Centromere B
The device CENPB.CE is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against Centromere B autoantigens in human plasma and sera.
Autoimmunity is the failure of an organism to recognize its own constituent parts as self, which allows an immune response against its own cells and tissues. Any disease that results from such an aberrant immune response is termed an Autoimmune Disease.
Rheumatoid autoimmune diseases are often associated with the occurrence of autoantibodies against several nuclear or cytoplasmatic antigens. We can to distinguish between Anti Nuclear Antibodies (ANA), associate with autoimmune systemic diseases as SLE (Systemic Lupus Erythematosus), RA (Reumatoid Arthritis), Scleroderma, MCDT (Mixed Connective Tissue Disease) and Sjögren’s Syndrome; and Extractable anti Nuclear Antibodies (ENA), associate with Polymyositis, SLE, MCDT and Sjogren’s Syndrome.
CENP-B is a nuclear antigen: autoantibodies against CENPB are clinically important since they are associated with Scleroderma or CREST Syndrome (Calcinosis, Raynaud phenomenon, Esophageal dysmotility, Sclerodactyly and Telangiectasia). Up to 70% of all CREST patients exhibit anti- Centromere B autoantibodies. Also in patients suffering from Primary Biliary Cirrhosis (PBC) anti-Centromere B antibodies can be found (10-20%).
Microplates are coated with a preparation of recombinant Centromere B antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-CENPB IgG are captured, if present, by the solid phase.
After washing out all the other components of the sample, in the 2nd incubation bound anti-CENPB are detected by the addition of anti hIgG antibody, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-CENPB IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.
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