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Human TNF-α ELISA Kit

The Human TNFα ELISA is to be used for the in-vitro quantitative determination of Tumor Necrosis Factor in human serum, plasma, buffered solutions or cell culture medium.


Species: Human
Catalog No Size
Product Catalog No: 950.090.192 Pack Size: 2 x 96

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Summary

TNFα may play a significant role in the pathogenesis of inflammatory disease of the joints and other tissues. Chin et al. found that TNFα , along with gamma interferon and IL-1 increased cell surface expression of ICAM -1 on synovial fibroblasts. Alvaro-Garcia et al. report that TNFα stimulates synovial proliferation.

Waage et al. found that increased levels of TNFα in patients with septicemia and meningococcal disease correlated with fatal outcome. Scuderi et al. suggest that increased levels of this cytokine may play a role in the host defense mechanism against parasitic infections. Girardin et al. reported that increased serum TNFα levels correlated with the number of risk factors involved in children with gram-negative sepsis and purpura fulminians. Elevated levels of TNFα were also found in individuals suffering from myocarditis .

Test Principle

A monoclonal antibody specific for TNFα has been coated onto the wells of the microtiter strips provided.

During the first incubation, TNFα present in the sample or standard and a monoclonal anti TNFα antibody conjugated to biotin are simultaneaously incubated.

Following incubation unbound biotinylated anti-TNFα is removed during a wash step.

Streptavidin-HRP is added and binds to the biotinylated anti TNF α. After incubation and a wash step a substrate solution reactive with HRP is added to the wells.

A coloured product is formed in proportion to the amount of TNFα present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm.

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