Human TGF-β1 ELISA Kit
The TGF-β1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human transforming growth factor beta-1 levels in cell culture supernatants, human serum, plasma, or other body fluids.
TGF-β1 is the first recognized transforming growth factor , its subunits of each 12.5 kDa are bound via disulphide bridges. TGF-β1 is inhibitive to T- and B cell proliferation as well as to maturation and activation of macrophages. It furthermore inhibits activity of natural killer cells and lymphokine activated killer cells and blocks production of cytokines.
Measurement of TGF-β1 in blood has been advocated for diagnosis of various diseases. TGF-β1 has been shown to be an organizer of responses to neurodegeneration .
An anti-human TGF-β1 coating antibody is adsorbed onto microwells.
Human TGF-β1 present in the sample or standard binds to antibodies adsorbed to the microwells.
A biotin-conjugated anti-human TGF-β1 antibody is added and binds to human TGF-β1 captured by the first antibody.
A coloured product is formed in proportion to the amount of human TGF-β1 present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm.