Human p53 ELISA Kit
The p53 ELISA is to be used for the in-vitro quantitative determination of p53 in serum, plasma, or cell lysates.
The p53 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for p53 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known p53 concentrations and unknowns are pipetted into these wells.
During the first incubation, the p53 antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for p53 is incubated. Then the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which actes on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of p53 present in the samples.
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