Human IL-7 ELISA Kit
The Human IL-7 ELISA is to be used for the in-vitro quantitative determination of interleukin-7 (IL-7) in human serum, plasma, buffered solutions or cell culture medium.
The IL-7 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-7 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-7 concentrations, control specimens and unknowns are pipetted into these wells.
During the first incubation, the IL-7 antigen and a biotinylated monoclonal antibody specific for IL-7 are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-7 present in the samples.
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