1. Home
  2. Research Topics
  3. Immunology
  4. Human IL-6 ELISA

Human IL-6 ELISA

The IL-6 ELISA is to be used for the in-vitro quantitative determination of interleukin-6 (IL-6) in human serum, plasma, buffered solutions or cell culture medium


Species: Human
Catalog No Size
Product Catalog No: 950.030.192 Pack Size: 2 x 96

Pack Size:
Pack Size:
Pack Size:
Pack Size:

Category:
Summary

Interleukin-6 (IL-6) is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis and may play a central role in host defense mechanisms (13, 31). The gene for human IL-6 has been localized to chromosome 7p21 (1). The genomic sequence has been determined (36). IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines (28), lipopolysaccharide (25) or viral infections (3). The IL-6 gene product is a single chain protein with a molecular mass ranging from 21 to 28 kDa, depending on the cellular source. Extensive posttranslational modifications like N- and O-linked glycosylation (20) as well as phosphorylation (21) seem to account for this heterogeneity. The cDNA for IL-6 predicts a precursor protein of 212 amino acids (10). IL-6 is a pleiotropic cytokine produced by a variety of cells. It acts on a wide range of tissues, exerting growthinduction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells.

Test Principle

A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided.

During the first incubation, IL-6 present in the sample or standard and a monoclonal anti IL-6 antibody conjugated to biotin are simultaneaously incubated.

Following incubation unbound biotinylated anti-IL-6 is removed during a wash step.

Streptavidin-HRP is added and binds to the biotinylated anti IL-6. After incubation and a wash step a substrate solution reactive with HRP is added to the wells.

A coloured product is formed in proportion to the amount of IL-6 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm.

References
  1. Kube D. et al., Blood, 2001; 98(3): 762 – 770
  2. Capo, X. et al., Nutrients,2016; 8(10).
  3. Ehlermann, P. et al.,Cardiovasc Diabetol.,2006; 5: 6
  4. Gombocz, K. et al., Crit Care,2007; 11(4): R87
  5. Nebor, D. et al., Haematologica,2011;96(11):1589-1594
  6. Shpacovitch V.M. et al., J. Leukoc. Biol., 2004; 76(2): 388 – 398
  7. Burger, R. et al., Hematol J.,2001; 2(1): 42-53.
  8. Corbi P. et al., Eur J Cardiothorac Surg., 2000; 18(1): 98 – 103
  9. Dore, P. et al., Clin Exp Immunol.,1997; 107(1): 182-8
  10. Gougelet, A. et al., PLoS One,2009; 4(11): e8026
  11. Le Meur Y. et al., Nephrol. Dial. Transplant., 1999; 14(10) : 2420 – 2426
  12. Puthier, D. et al., Blood,1996; 88(12): 4659-66
  13. Corvaisier, M. et al., PLoS Biol.,2012;10(9): e1001395
  14. Cunin, P. et al., J. Immunol.,2011;186(7): 4175-4182.
  15. Forsbach, A. et al., J. Immunol.,2008;180(6): 3729-3738.
  16. Kerr J. et al., J. Gen. Virol., 2001; 82(Pt 12): 3011-3019
  17. Liao, B. et al., Emerg Microbes Infect.,2015; 4(4): e24
  18. Nagao, Y. et al., Malar J.,2008; 7: 113.
  19. Stebbing, J. et al., Clin Exp Immunol.,2004; 138(2): 312-6.
  20. Tang, Y. et al., Am J Trop Med Hyg.,2008; 79(2): 154-158.
  21. Tang, Y. et al.,PLoS One,2010; 5(12): e15631
  22. Vollmer, J. et al.,Immunology,2004; 113(2): 212-23.
  23. Zhang, Y. et al., Lupus, 2011; 20: 1172 – 1181
  24. Chavushyan, A. et al., Schizophr Res Treatment, 2013: 125264
  25. Cutolo M. et al., Ann. Rheum. Dis, 2005; 65(6): 728-35
  26. Cutolo, M. et al., Arthritis Res Ther.,2009;11(6): R176
  27. Eilertsen, G. O. et al., Lupus,2011 ; 20(6): 607-613.
  28. Kwon K.Y. et al., J. Korean Med. Sci., 2001; 16(6): 774 – 780
  29. Lu, C. et al., Lupus, 2015;24(1):18-24
  30. Mochel, F. et al., PLoS One,2007; 2(7): e647.
  31. Mohamed, H. S. and M. M. Meguid, Saudi J Anaesth.,2017;11(1): 9-14.
  32. Suzuki, N. et al., Tob Induc Dis.,2016; 14: 20.
  33. Tuttolomondo, A. et al.,PLoS One,2016; 11(12): e0165443
  34. Azadbakht, L. et al., Diabetes Care,2007;30(4):967-73
  35. Rybka, J. et al.,Clin Exp Med.,2015; 16(4): 493-502.
  36. Akbari, F. et al., Iran J Neurol.,2016; 15(2): 75-9.
  37. Akram Kooshki, A. et al.,Glob J Health Sci.,2015; 7(7 Spec No): 1-5
  38. Auf, G. et al., PNAS,2010;0914072107
  39. Badoual, C. et al., Cancer Res.,2008; 68(10): 3907-3914.
  40. Balabanian K. et al., Am. J. Respir. Crit. Care Med., 2002; 165(10): 1419 – 1425
  41. Barber, M. D. et al., Br J Cancer,2004 ; 90(6): 1129-32
  42. Biswas, S. et al.,Korean J Intern Med.,2010 ; 25(1): 44-50
  43. Bonnans, C. et al., Am J Pathol., 2006; 168 (4) :1064-72.
  44. Bozic, M. et al.,PLoS One,2015;10(8): e0136863
  45. Calabro, M. L. et al., Blood,2009; 113(19): 4525-4533.
  46. Capo, X. et al., Oxid Med Cell Longev., 2015: 187849
  47. Chabbert-de Ponnat, I. et al., Int Immunol.,2005; 17(4): 439-47.
  48. Chang K-T. et al., Am J Physiol Lung Cell Mol Physiol, 2005; 289(3): L438-45
  49. Chang, K. T. et al., Am J Physiol Lung Cell Mol Physiol.,2005; 289(3): L446-53.
  50. Chen, Y. C. et al., Mediators Inflamm., 2010: 573594
  51. Coles, J. G. et al., J Thorac Cardiovasc Surg.,2005;129(5): 1128-36.
  52. Cot, M. et al.,PLoS One,2011; 6(10): e26316
  53. Cutolo M. et al., Ann. Rheum. Dis, 2005; 64(2): 212 – 216
  54. de Gonzalo-Calvo, D. et al., Age (Dordr),2011; 34(3): 761-71
  55. de Haan, J. J. et al.,Crit Care,2009; 13(3): R86
  56. Faam, B. et al., Iran J Basic Med Sci.,2014;17(8): 577-82
  57. Fu, J. F. et al.,World J Gastroenterol.,2009; 15(8):912-8
  58. Garcia-Posadas, L. et al., Invest. Ophthalmol. Vis. Sci., 2013 ; 54(10): 7143-7152
  59. Garcia-Posadas, L. et al., PLoS One,2017; 12(3): e0171099
  60. Gehrke, T. et al., Oncol Lett.,2016; 12(5): 3549-3554
  61. Giamarellos-Bourboulis, E. J. et al., PLoS One,2009; 4(12): e8393
  62. Giamarellos-Bourboulis, E. J. et al., Crit Care,2006; 10(3): R76
  63. Gironella, M. et al., Gut ,2005; 54(9): 1244-53.
  64. Gomez-Tortosa, E. et al., Arch Neurol., 2003; 60(9): 1218-22
  65. Guerrero, A. et al., Aging Cell,2015; 14(2): 274-83
  66. Gulati, S. et al.,Am J Reprod Immunol, 2012; 67(3): 235-40
  67. Guldiken, B.et al., Angiology,2008; 59(2): 224-229.
  68. Gupta, A. et al., Human and Experimental Toxicology,2011;30(10): 1445-1453
  69. Hjuler Nielsen, M. et al., PLoS One,2015;10(4): e0121516
  70. Jego, G. et al., Blood, 1999; 94(2): 701-12
  71. Jouan, J. et al., J. Thorac. Cardiovasc. Surg., 2012;144(2):467-4732
  72. Kanakdande, V. et al.,Contemp Clin Dent.,2015; 6(3): 348-57
  73. Kashyap, B. et al., J Trop Pediatr.,2011:fmr093
  74. Koussoulas, V. et al.,World J Gastroenterol.,2006; 12(41): 6711-4
  75. Kruger, K. et al., J Appl Physiol.,2011; 110(5): 1226-1232
  76. Kulms D. et al., J. Biol. Chem., 2000; 275(20): 15060 – 15066
  77. Kumpf, O. et al. ,Crit Care,2010; 14(3): R103.
  78. Kyriakopoulou, M. et al., Mediators Inflamm., 2008: 450196.
  79. Lee, H. W. et al., Ann Dermatol.,2013 ; 25(2): 173-80
  80. Lekkou A. et al., Clin. Diagn.Lab. Immunol., 2004; 11(1):161 – 167
  81. Lewis, S. et al., Respir Res.,2009 ; 10: 44
  82. Lieblein, J. C. et al.,BMC Cancer,2008; 8: 302
  83. Loo, W. T. et al., J Transl Med.,2012; 10 Suppl 1: S8
  84. Lotrich, F. E. et al., Brain Behav Immun .,2013;31: 48-53.
  85. Mahalle, N. et al.,Indian J Endocrinol Metab.,2014 ; 18(5): 708-14
  86. Mahalle, N. et al., Indian J Endocrinol Metab.2014 ; 18(1): 48-55
  87. Mahalle, N. et al., Indian J Endocrinol Metab.2013; 17(5): 844-50
  88. Mahalle, N. et al., Ann Med Health Sci Res., 2014; 4(5): 706-12
  89. Montes, C. L. et al.,Cancer Res.,(2008; 68(3): 870-879.
  90. Moreaux, J. et al., Blood,2011; 117(4): 1280-1290
  91. Mori, T. et al., Exp Anim.,2016; 65(4): 455-463.
  92. Musleh, G. S. et al.,Eur. J. Cardiothorac. Surg.,2009; 35(3): 511-514.
  93. Netam, R. et al., Indian J Med Res.,2015; 141(6): 775-82
  94. Onambele-Pearson, G. L. et al., Age (Dordr),2010; 32(2): 139-53
  95. Onambele-Pearson, G. L. et al.,Age (Dordr),2010; 32(2): 125-38
  96. Onambele-Pearson, G. L. and S. J. Pearson, Age (Dordr),2012;34(2): 427-38
  97. Pac-Kozuchowska, E. et al., Med Sci Monit.,2016;22: 1534-9.
  98. Paduch, R. and A. Wozniak, J Ophthalmic Vis Res.,2015; 10(3): 229-37
  99. Paduch, R., et al., Balkan Med J.,2014 ; 31(1): 29-36
  100. Panes, J. et al., Am J Physiol Gastrointest Liver Physiol.,2007; 293: G739 – G748
  101. Pasi, F. et al., Anticancer Res.,2010; 30(7): 2769-2772
  102. Pelekanou, A. et al.,Crit Care,2009; 13(6): R172
  103. Peng, L. S. et al.,World J Emerg Med.,2015 ; 6(2): 123-30
  104. Perret D. et al., J. Biol. Chem., 2004; 279(42): 43961 – 43970
  105. Perry, M. G. et al., Rheumatology(Oxford),2006;45(2): 229-30.
  106. Pitta, M. G. et al., J Clin Invest.,2009; 119(8): 2379-87
  107. Prakash, S. et al., Clin Orthop Relat Res.,2013; 471(7): 2340-6
  108. Prather, A. A. et al.,Brain Behav Immun., 2009;23(8): 1109-16
  109. Rajappa, M. et al., Angiology,2009; 60(4): 419-426.
  110. Rana, S. V. et al., J Crohns Colitis,2014 ; 8(8): 859-865
  111. Rasool, R. et al., Asia Pac Allergy,2014 ; 4(4): 206-11
  112. Robertson, M. D. et al., J. Clin. Endocrinol. Metab.,2012; 97(9): 3326-3332.
  113. Rotter V. et al., J. Biol. Chem., 2003; 278(46): 45777 – 45784
  114. Routsi, C. et al.,Clin Exp Immunol.,2005;142(1): 62-7.
  115. Sell, H. et al., Am J Physiol Endocrinol Metab., 2008;294:E1070-E1077
  116. Shadman, Z. et al., J Diabetes Metab Disord.,2013; 12(1): 42
  117. Shetty, P. et al., Annals of Clinical Biochemistry,2016: 0004563216665867
  118. Sopasakis V. et al., Obes. Res., 2004; 12(3): 454 – 460
  119. Soriano-Izquierdo A. et al., J. Leukoc. Biol., 2004; 75(2): 214 – 223
  120. Souza, L. S. et al., J Rheumatol.,2008; 35(11): 2265-2271.
  121. Stebbing J. et al., Ann. Onc., 2003; 14(11): 1660 – 1666
  122. Stefansson, B. V. et al., Nephron Extra,2012; 2(1): 55-65.
  123. Sun, Y. et al., J. Immunol.,2008; 180(6): 4173-4181.
  124. Sun, Z. et al.,Cent Eur J Immunol.,2014; 39(2): 216-22
  125. Sureda, A. et al., PLoS One,2016; 11(9): e0163371.
  126. Szumowska, A. et al., Clinical and Applied Thrombosis/Hemostasis,2016; 22(7): 679-684
  127. Tabibi, H. et al., Perit. Dial. Int.,2016; 36(2): 140-145
  128. Tayal, D. et al.,Indian J Clin Biochem.,2014; 29(2): 139-44
  129. Tolahunase, M. et al., Oxid Med Cell Longev.,2017; 2017: 7928981.
  130. Toutirais, O. et al.,Clin Exp Immunol.,2007 ; 149(2): 372-7
  131. Triantafilou K. et al., J. Cell Sci., 2001; 114(Pt 13): 2535 – 2545
  132. Tsaganos, T. et al.,BMC Infect Dis.,2006; 6: 142.
  133. Tuttolomondo, A. et al., Cardiovasc Diabetol.,2010; 9: 50
  134. van Setten, P. A. et al., Blood,1996; 88(1): 174-83.
  135. Verghese, B. et al., Indian J Clin Biochem.,2011 ; 26(4): 373-7
  136. Versaci, F. et al., Mayo Clin Proc.,2012; 87(1): 50-8
  137. Virdis, A., et al.,Eur. Heart J.,2015: ehv365
  138. Vita N. et al., J. Immunol., 1997; 158(7): 3457 – 3462
  139. Vollmer J. et al., Antimicrob. Agents Chemother., 2004; 48(6): 2314 – 2317
  140. Vollmer J. et al., J Leukoc Biol., 2004;76(3) : 585 – 593
  141. Walavalkar, V. et al., Eur J Cardiothorac Surg.,2016; 49(5): 1403-1410
  142. Williamson, P. A. et al., Eur. Respir. J.,2011; 37(1): 206-209
  143. Wong C.K. et al., Am. J. Respir. Cell Mol. Biol., 2005; 33(2): 186 – 194
  144. Wrenger, S. et al., J. Leukoc. Biol.,2006; 80(3): 621-629.
  145. Yadav, U. C. S. et al., J. Immunol.,2009; 183(7): 4723-4732.
  146. Yadav, U. C. et al., PLoS One,2009; 4(8): e6535
  147. Yadav, U. C. et al., Chem Biol Interact.,2011; 191(1-3): 339-45
  148. Akbarpour, M.,Biol Sport,2013;30(1): 21-7
  149. Rai, G. et al.,Ann Lab Med., 2018 Mar;38(2):125-131
  150. Mokhtare, M. et al.,Middle East J Dig Dis. 2017 Oct;9(4):228-234.
  151. Unal,D. et al.,Asia Pac Allergy, 2017;7:74-81
  152. Mateen,S. et al.,PLoS One. 2017 Jun 8;12(6):e0178879
  153. Tolahunase, M. et al., Oxid Med Cell Longev. 2017;2017:2784153
  154. Arora,V. et al.,J Anaesthesiol Clin Pharmacol. 2017 Jan-Mar;33(1):57-63
  155. Dineshkumar, T. et al.,Asian Pac J Cancer Prev. 2016 Nov 1;17(11):4899-4906.
  156. Turkoglu, R. et al.,Med Princ Pract. 2014;23(3):239-45.
  157. Zhang, J. et al.,Tanaffos. 2017 Jun;16(4):260-269.
  158. Valdor, R. et al.,Oncotarget. 2017 Aug 2;8(40):68614-68626.
  159. Kurowski, M. et al.,Arch Med Sci. 2018 Jan;14(1):60-68.
  160. Krasimirova,E. et al., World J Exp Med., 2017 Aug 20;7(3):84-96.
  161. Shete, S.U. et al.,J Educ Health Promot. 2017 Aug 9;6:76
Order Enquiry

    Order Enquiry Form

    Documents
    Enquiry