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Human IL-1β ELISA Kit

The Human IL-1β ELISA is to be used for the in-vitro quantitative determination of interleukin-1 beta (IL-1β) in human serum, plasma, buffered solutions or cell culture medium.


Species: Human
Catalog No Size
Product Catalog No: 850.006.192 Pack Size: 2 x 96

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Test Principle

The IL-1β Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-1β has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-1β concentrations and unknowns are pipetted into these wells.

During the first incubation, the IL-1β antigen and a biotinylated monoclonal antibody specific for IL-1β are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1β present in the samples.

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