1. Home
  2. Research Topics
  3. Immunology
  4. Human IL-1β ELISA Kit

Human IL-1β ELISA Kit

The Human IL-1β ELISA is to be used for the in-vitro quantitative determination of interleukin-1 beta (IL-1β) in human serum, plasma, buffered solutions or cell culture medium.


Species: Human
Catalog No Size
Product Catalog No: 850.006.096 Pack Size: 1 x 96

Pack Size:
Pack Size:
Pack Size:
Pack Size:

Category:
Test Principle

The IL-1β Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-1β has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-1β concentrations and unknowns are pipetted into these wells.

During the first incubation, the IL-1β antigen and a biotinylated monoclonal antibody specific for IL-1β are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1β present in the samples.

References
  1. Corvaisier, M. et al., PLoS Biol.,2012;10(9): e1001395
  2. Cunin, P. et al., J. Immunol.,2011;186(7): 4175-4182.
  3. Kerr J. et al., J. Gen. Virol., 2001; 82(Pt 12): 3011-3019
  4. Miot, C. et al., Gut,2014: gutjnl-2013-306604
  5. Montero M.T. et al., J. Immunol., 2004; 173(8): 4936 – 4944
  6. Nagao, Y. et al., Malar J.,2008; 7: 113.
  7. Loo, W. T. et al., J Transl Med.,2012;10 Suppl 1: S8
  8. Beyth S. et al., Blood, 2005; 105(5): 2214 – 2219
  9. Buenestado, A. et al., J Parenter Enteral Nutr.,2006;30(4):286-96
  10. Castroflorio, T. et al., Angle Orthod.,2016;DOI:10.2319/022416-159.1
  11. Chavushyan, A. et al., Schizophr Res Treatment, 2013: 125264
  12. Cutolo M. et al., Ann. Rheum. Dis, 2005; 65(6): 728-35
  13. Cutolo, M. et al., Arthritis Res Ther.,2009;11(6): R176
  14. Diaw, M. et al., Diabetes Care,2015; 38(11): 2120-2127
  15. Eilertsen, G. O. et al., Lupus,2011 ; 20(6): 607-613.
  16. Fornander, L. et al., PLoS One,2013; 8(12): e83089
  17. Gomez-Tortosa, E. et al., Arch Neurol.,2003;60(9):1218-22
  18. Kwon K.Y. et al., J. Korean Med. Sci., 2001; 16(6): 774 – 780
  19. Lapinet J.A. et al., Infect Immun.,2000; 68(12):6917-23
  20. Lewis, S. et al., Respir Res.,2009; 10: 44
  21. Lietzen, N. et al.,PLoS Pathog.,2011 ; 7(5): e1001340
  22. Lu, C. et al., Lupus, 2015;24(1):18-24
  23. Markiewicz, L. et al.,Biomed Res Int.,2015; 2015: 812503
  24. Mochel, F. et al., PLoS One,2007; 2(7): e647.
  25. Mohamed, H. S. and M. M. Meguid, Saudi J Anaesth.,2017;11(1): 9-14.
  26. Ohman, T. et al., J. Immunol.,2014 ;192(12): 5952-5962
  27. Paduch, R. and A. Wozniak J Ophthalmic Vis Res.,2015; 10(3): 229-37
  28. Paduch, R. et al.,Balkan Med J.,2014;31(1): 29-36
  29. Rajbhoj, P. H. et al.,J Clin Diagn Res.,2015; 9(2): CC01-5
  30. Shadman, Z. et al., J Diabetes Metab Disord.,2013; 12(1): 42
  31. Suzuki, N. et al., Tob Induc Dis.,2016; 14: 20.
  32. Tantibhedhyangkul, W. et al.,PLoS Negl Trop Dis.,2011; 5(5): e1028.
  33. Tuttolomondo, A. et al.,PLoS One,2016; 11(12): e0165443
  34. Zhou, C. et al., J Neuroinflammation,2015; 12(1): 200
  35. Fernandez-Lizarbe, S. et al., J. Immunol.,2009; 183(7): 4733-4744.
  36. Rai, G. et al.,Ann Lab Med., 2018 Mar;38(2):125-131
  37. Mokhtare, M. et al.,Middle East J Dig Dis. 2017 Oct;9(4):228-234.
  38. Barska,K. et al.,PLoS One, 2017; 12(7): e0181533.
  39. Kurowski, M. et al.,Arch Med Sci. 2018 Jan;14(1):60-68.
Order Enquiry

Order Enquiry Form

Documents
Enquiry