Human Anti-Mullerian Hormone
This test kit is intended for use in the quantitative determination of human Anti-Mullerian Hormone (AMH) levels in serum lithium heparin plasma samples. It is for in-vitro diagnostic use only.
Anti-Müllerian Hormone or Müllerian-inhibiting hormone (MIH) is a glycoprotein hormone structurally related to inhibin and activin from the transforming growth factor beta superfamily, whose key roles are in growth differentiation and folliculogenesis. AMH expression is critical to sex differentiation at a specific time during fetal development, and appears to be tightly regulated by nuclear receptor SF1, transcription GATA factors, sex-reversal gene DAX1, and follicle-stimulating hormone (FSH). AMH is activated by SOX9 in the Sertoli cells of the male fetus thereby arresting the development of fallopian tubes, uterus, and upper vagina. AMH is also a product of granulosa cells of the preantral and small antral follicles in women. As such, AMH is only present in the ovary until menopause. AMH level is also lower and even below the detection limit if women with premature ovarian failure of any cause, including after cancer chemotherapy, etc.
This ELISA is designed, developed and produced for the quantitative measurement of human Anti-Mullerian Hormone in serum or heparin plasma samples. The assay utilizes the two-site “sandwich” technique with two selected antibodies that bind to different epitopes of human AMH.
Assay calibrators, controls and patient samples are added directly to microtiter wells of a microplate that is coated with antibody to N-terminal AMH along with another AMH specific antibody labelled with horseradish peroxidase (HRP). After an initial incubation period, the plate is washed and a “sandwich” of solid-phase antibody – human AMH – HRP-conjugated monoclonal antibody is formed. The unbound monoclonal antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human AMH in the test sample. A standard curve is generated by plotting the absorbance versus the respective human AMH concentration for each standard on a Cubic or point-to-point curve fitting. The concentration of human AMH in test samples is determined directly from this calibration curve.
- Lee, M. et al (1993); Müllerian-inhibiting substance: A gonadal hormone with multiple functions. Endocrine Reviews, 142, 152-164.
- Hudson, et al (1990); An immunoassay to detect human Müllerian inhibiting substance in males and females during normal development. Journal of Clinical Endocrinology and Metabolism, 70, 16-22.
- Lee, M et al (1996); Müllerian Inhibiting Substance in human: normal levels from infancy to adulthood. Journal of Clinical Endocrinology and Metabolism 81, 571 – 575.