HTLV I/II

This HTLV-I/II enzyme-linked immunosorbent assay (ELISA) kit employs a technique called a qualitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with synthetic HTLV-I /II peptides. Samples or controls are added to the microtiter plate wells and incubated. HTLV l/ll specific antibodies, if present, will bind the antigenic determinant of the HTLV l/ll peptides and become immobilized by the antigen pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any HTLVI/ II antibodies and other components of the sample. A standardized preparation of horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibodies is added to each well to “sandwich” the HTLV-I/II antibodies immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3′,5,5′ tetramthyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells containing HTLV-I/II specific antibodies and enzymeconjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm. Samples with O.D values greater than or equal to the Cut-off Value are considered reactive by the criteria of this HTLV-I/II Antibody ELISA Kit.