HSV1&2 IgG
Enzyme ImmunoAssay (ELISA) for the quantitative/qualitative determination of IgG antibodies to Herpes Simplex Virus type 1 and 2 in human plasma and sera.
Herpes Simplex Virus type 1 (HSV1) and type 2 (HSV2) are large complex DNA-containing viruses which have been shown to induce the synthesis of several proteins during infection, possessing an high number of crossreactive determinants and just a few of type-specific sequences.
The majority of primary and recurrent genital herpetic infections are caused by HSV2; while non genital infections, such as common cold sores, are caused primarily by HSV1. The detection of virus specific IgG and IgM antibodies are important in the diagnosis of acute/primary virus infections or reactivations of a latent one, in the absence of evident clinical symptoms.
Asymptomatic infections may happen for HSV in apparently healthy individuals and during pregnancy. Severe herpetic infections may happen in immunocompromised and suppressed patients in which the disease may evolve toward critical pathologies.
The determination of HSV specific antibodies has then become important in the monitoring of “risk” patients and in the follow up of acute and severe infections.
Microplates are coated with native inactivated HSV1 and HSV2. The solid phase is first treated with the diluted sample and IgG to HSV are captured, if present, by the antigens.
After washing out all the other components of the sample, in the 2nd incubation bound anti HSV IgG are detected by the addition of polyclonal specific anti hIgG antibodies, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti HSV IgG antibodies present in the sample. A Calibration Curve, calibrated against an internal Gold Standard, makes possible a quantitative determination of the IgG antibody in the patient.
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