HPV IgG ELISA
Enzyme ImmunoAssay (ELISA) for the semi-quantitative determination of IgG class antibodies to Human Papilloma Virus (or HPV) in human plasma and sera. The product is supplied for research purpose only. It is not for use in the diagnosis or for the follow-up of patients administered with the vaccines containing HPV antigens.
Human Papilloma Viruses are double stranded DNA organisms, without envelope, bearing to the group of Papovavirus. HPV infects epithelial cells and are associated with benign and malign lesions as papillomas, condilomas and carcinomas. Human Papilloma Viruses are pretty heterogenic and are classified in several types that include high-risk oncogenic types (16,18,31,33,35,39,45,51,52,56,58,59,68) and low risk non oncogenic types. Synthetic antigens have been recently used to produce vaccines able to protect against infections due to the most carcinogenic strains of HPV, whose distribution has started in many countries of the world and whose real efficacy as vaccine is under field investigation.
Microplates are coated with recombinant VLP’s derived from HPV Type 6, 11, 16 e 18. In the 1st incubation, the solid phase is treated with diluted samples and anti-HPV IgG are captured, if present, by the antigens. After washing out all the other components of the sample, in the 2nd incubation bound anti-HPV IgG are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-HPV IgG antibodies present in the sample. A cut-off value turns the measured optical densities into positive or negative results.
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