Homoarginine
Homoarginine is a non-essential cationic amino acid, which is formed from lysine. In vitro and in vivo, homoarginine shows characteristics similar to arginine.
Only 20 Microlitre (µl) sample volume: EDTA-Plasma, serum or cell culture
Sample Preparation: Acylation, shake for 15 minutes at room temperature
ELISA: Shake for 140 – 150 minutes at room temperature
Homoarginine is a non-essential cationic amino acid, which is formed from lysine. In vitro and in vivo, homoarginine shows characteristics similar to arginine. Epidemiological investigations in two large independent cohorts, namely the German diabetes dialysis (4D) – study and the Ludwigshafen Risk and Cardiovascular Health (LURIC) – study have identified homoarginine as useful predictor of cardiovascular events and mortality.
Beyond that homoarginine concentrations are directly correlated with kidney function and are significantly associated with the progression of chronic kidney disease (CKD). Low homoarginine concentrations might be an early indicator of kidney failure and a potential target for the prevention of disease progression which needs further investigations. Furthermore homoarginine could be a useful marker for the monitoring of hemodialysis patients.
Cited: J Lab Med, 2011; 35 (3): 153–159
We offer a competitive Homoarginine-ELISA using the microtiter plate format. The correlation of the ELISA method to LC-MS is exceptionally good. No interferences with any therapeutic drugs are observed. The ELISA method allows the measurement of large series of patient samples.
Homoarginine as a biomarker for the risk of mortality is applied for a patent. EP2533653A1 and US20130143240.
The competitive Homoraginine-ELISA uses the microtiter plate format. Homoarginine is bound to the solid phase of the microtiter plate. Homoarginine in the samples is acylated and competes with solid phase bound Homoarginine for a fixed number of rabbit anti-Homoarginine antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase Homoarginine is detected by antirabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase Homoarginine is inversely proportional to the Homoarginine concentration of the sample.
- A. Meinitzer, Ch Drechsler. A. Tomaschitz, S. Pilz, V. Krane, Ch. Wanner, W. März Homoarginin, ein neuer kardiovaskulärer Risikomarker bei Dialysepatienten J. Lab. Med. 2011, 35 (3): 153 -159, copy 2011 by Walter de Gruyter, Berlin, Boston
- W. März A. Meinitzer, Ch. Drechsler, S. Pilz, V. Krane, M.E. Kleber, J. Fischer, B.R. Winkelmann, B.O. Böhm, E. Ritz, Ch. Wanner. Homoarginine, Cardiovascular Risk and Mortality Circulation 2010, 122: 967-975
- Pietro Ravani, Renke Maas, Fabio Malberti, Paola Pecchini, Maren Mieth, Robert Quinn, Giovanni Tripepi, Francesca Mallamaci, Carmine Zoccali Homoarginine and Mortality in Pre-Dialysis Chronic Kidney Disease (CKD) Patients Plos One; September 2013, Volume 8, Issue 9: 1-6
- Ch. Drechsel, B. Kolleritz. A, Meinitzer, W. März, E. Ritz, P. König, U. Neyer, S. Pilz, Ch. Wanner, F. Kronenberg Homoarginine and Progression of Chronic Kidney Disease: Results from the Mild to Moderate Kidney Disease Study May 2013; Plos One, 10, 1371
- A.A. Khalil, D. Tsikas, R. Akolekar, J. Jordan, K.H. Nicolaides Asymmetric dimethylarginine, arginine and homoarginine at 11-13 weeks´ gestation and preeclampsia: a case control study. J. of Human Hypertension January 2013 27; 38-43
- A. Jazwinska-Kozuba, J. Martens-Lobenhoffer, O. Kruszelnicka, J. Rycaj, B. Chyrchel, A. Surdacki, S. M. Bode-Böger Opposite Associations of Plasma Homoarginine and Ornithine with Arginine in Healthy Children and Adolescents Int. J. Mol. Sci. 2013, 14, 21819-21832
- Choe CU, Atzler D, Wild PS, Carter AM, Böger RH, Ojeda F, Simova O, Stockebrand M, Lackner K, Nabuurs C, Marescau B, Streichert T, Müller C, Lüneburg N, De Deyn PP, Benndorf RA, Baldus S, Gerloff C, Blankenberg S, Heerschap A, Grant PJ, Magnus T, Zeller T, Isbrandt D, Schwedhelm E Homoarginine levels are regulated by L-arginine: glycine amidinotransferase and affect stroke outcome; results from human and murine studies Circulation, 2013 Sep 24, 128 (13) 1451-1461
- van der Zwan, L., Davids, M., Scheffer, P.; et al. L-Homoarginine and L-arginine are antagonistically related to blood pressure in an elderly population: the Hoorn study Journal of Hypertension 2013: 31:1114–1123