Hemoglobin A1c Assay Kit (HbA1c)
For quantitative determination of the HbA1c /Total Hemoglobin ratio (%) in human blood.
The concentration of HbA1c is representative of the mean blood glucose level over the preceding four to eight weeks. HbA1c in the blood of diabetic patients increases with rising blood glucose levels. Therefore it is described as a long term indicator of diabetic control unlike blood glucose which is only a short term indicator of diabetic control. HbA1c test can screen for and diagnose diabetes or risk of developing diabetes. It helps to evaluate how well the person’s glucose levels have been controlled by treatment over time. This test is also used as a measure of risk for the development of gestational diabetes and diabetes complications.
This method utilizes the interaction of antigen and antibody to directly determine the HbA1c in whole blood. Total hemoglobin and HbA1c have the same unspecific absorption rate to latex particles. When mouse anti-human HbA1c monoclonal antibody is added (R2), latex-HbA1c-mouse anti human HbA1c antibody complex is formed. Agglutination is formed when goat anti-mouse IgG polyclonal antibody interacts with the monoclonal antibody. The measured absorbance is proportional to the HbA1c absorbed on to the surface of latex particals, which in turn is proportional to the percentage of HbA1c in the sample.
- Goldstein DE, Little RR, Lorenz RA, et al. Tests of glycemia in diabetes. Diabetes Care 1995;18:896909.
- The Diabetes Control and Complications Trial Research Group. The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus.N EnglJ Med 1993;329:977-986.
- American Diabetes Association, “Standards of Medical Care for Patients with Diabetes Mellitus”, (Position statement), Diabetes Care, 21 (Suppl.1): S23 S31(1998).
- Pantheghini M, John WG on behalf of the IFCC Scientific Division. Implementation of haemoglobin A1c results traceable to the IFCC reference system: the way forward. Clin Chem Lab Med 2007; 45(8): 942-4.