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HCG ß free

The Free β-HCG ELISA is an enzyme immunoassay for the quantitative in vitro measurement of free beta subunit of human chorionic gonadotropin (free β-hCG) in serum. In the United States, this kit is intended for Research Use Only.


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Product Catalog No: EIA-4718 Pack Size: 96 Wells

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Summary

Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone normally produced by placenta during pregnancy. The hormone is present in blood and urine around seven to thirteen days following implantation of the fertilized ovum. Structurally intact hCG molecules consist of two non-covalently linked polypeptide subunits, the alpha and beta chain subunits. Measurement of intact hCG and of the alpha subunit of hCG appears to give similar results in blood and urine but not the levels of beta subunit.

Test Principle

The Free β-HCG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on a Free β-hCG molecule. An aliquot of patient sample containing endogenous Free β-hCG is incubated in the coated well with enzyme conjugate, which is an anti-β-HCG antibody [rabbit] conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of Free β-HCG in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of Free β-HCG in the patient sample.

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References
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