HBe Ag/Ab ELISA
Enzyme ImmunoAssay (ELISA) for the determination of Hepatitis B Virus "e" Antigen and Antibody in human plasma and sera.
HBeAg:
HBeAg, if present in the sample, is captured by a specific monoclonal antibody, in the 1st incubation. In the 2nd incubation, after washing, a tracer, composed of a mix of two specific anti HBeAg monoclonal antibodies, labeled with peroxidase (HRP), is added to the microplate and binds to the captured HBeAg. The concentration of the bound enzyme on the solid phase is proportional to the amount of HBeAg in the sample and its activity is detected by adding the chromogen/substrate in the 3rd incubation. The presence of HBeAg in the sample is determined by means of a cut-off value that allows for the semiquantitative detection of the antigen.
HBeAb:
Anti HBeAg antibodies, if present in the sample, compete with a recombinant HBeAg preparation for a fixed amount of an anti HBeAg antibody, coated on the microplate wells. The competitive assay is carried out in two incubations, the first with the sample and recHBeAg, and the second with a tracer, composed of two anti HBeAg monoclonal antibodies, labeled with peroxidase (HRP). The concentration of the bound enzyme on the solid phase becomes inversely proportional to the amount of anti HBeAg antibodies in the sample and its activity is detected by adding the chromogen/substrate in the third incubation. The concentration of HBeAg specific antibodies in the sample is determined by means of a cut-off value that allows for the semi quantitative detection of anti HBeAg antibodies.
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