GM-CSF Receptor Alpha Chain (CD116) Human ELISA
GM-CSF is a small glycoprotein growth factor which stimulates the production and function of neutrophils, eosinophils and monocytes. It can be produced by a wide variety of tissue types. The biological effects of GM-CSF are mediated through its binding to cell surface receptors, which appear to be widely expressed by thermatopoietic cells and also by some non-thermatopoietic cells, such as endothelial cells.
Research topic
Cytokines and chemokines and related molecules
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
100 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
31.25–1000 pg/ml
Limit of Detection
< 16 pg/ml
– Prevost JM, Pelley JL, Zhu W, D’Egidio GE, Beaudry PP, Pihl C, Neely GG, Claret E, Wijdenes J, Brown CB. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and inflammatory stimuli up-regulate secretion of the soluble GM-CSF receptor in human monocytes: evidence for ectodomain shedding of the cell surface GM-CSF receptor alpha subunit. J Immunol. 2002 Nov 15;169 (10):5679-88