GM-CSF Human ELISA
GM-CSF is a small glycoprotein growth factor which stimulates the production and function of neutrophils, eosinophils and monocytes. It can be produced by a wide variety of tissue types. The biological effects of GM-CSF are mediated through its binding to cell surface receptors, which appear to be widely expressed by thermatopoietic cells and also by some non-thermatopoietic cells, such as endothelial cells.
Research topic
Cytokines and chemokines and related molecules
Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Amniotic fluid, Plasma, Cell culture supernatant
Sample Requirements
50 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
7.8–500 pg/ml
Limit of Detection
2.9 pg/ml
Intra-assay (Within-Run)
CV = 6.0%
Inter-assay (Run-to-Run)
CV = 4.4%
Spiking Recovery
85,00%
Dilutation Linearity
109,00%
– Kaiserova M, Grambalova Z, Otruba P, Stejskal D, Prikrylova Vranova H, Mares J, Mensikova K, Kanovsky P. Cerebrospinal fluid levels of chromogranin A and phosphorylated neurofilament heavy chain are elevated in amyotrophic lateral sclerosis. Acta Neurol Scand. 2017 Feb 10. doi: 10.1111/ane.12735