Glycated Serum Protein (Glycated Albumin)
Glycated Serum Protein Assay is intended for the quantitative determination of glycated serum proteins in serum.
enzymatic assay for glycated serum protein provides improved specificity and reliability compared to conventional NBT-based methods. The assay is highly precise with a total CV ≤ 1.3%. The assay offers extensive linearity up to 1354 μmol/L. The assay has no significant interference from: ascorbic acid (5 mg /dL); bilirubin (7.5 mg/dL); bilirubin conjugated (5 mg/dL); glucose (2400 mg/dL; hemoglobin (200 mg/dL); triglycerides (2000 mg/dL); and uric acid (35 mg/dL).
Glycated Serum Protein Assay uses proteinase K to digest GSP into low molecular weight glycated protein fragments (GPF), and uses Diazyme’s specific fructosaminase™, a microorganism originated amadoriase to catalyze the oxidative degradation of Amadori product GPF to yield PF or amino acids, glucosone and H2O2. The H2O2 released is measured by a colorimetric Trinder end-point reaction. The absorbance at 546-600 nm is proportional to the concentration of glycated serum proteins.
- Abidin D. et al. An Improved Enzymatic Assay for Glycated Serum Protein. Anal. Methods 2013; 5: 2461-2469