Glycated albumin Assay Kit (GA)
For the quantitative ratio determination of human glycated albumin(GA) and albumin(ALB) in serum or plasma. For in vitro diagnostic use only.
Glycation is the result of a sugar molecule,such as gluctose or fructose,binding to a protein or lipid molecule without the controlling action of an enzyme.It is a haphazard process that inpairs the function of biomecules.The high levels of glucose present in diabetes mellitus results in increased glycation of all protein, including albumin.So the glycated albumin assay may be used as a market of glycemic control in diabetes and reflects glycemic control over the previous 2 to 4weeks.
1. Measurement of GA Add protease which is specific to albumin into sample to decopose glycated albumin,producing glycated animo acid. Then glycated amino acid is translated into glucosone,animo acid,H2O2 by the function of glycated amino acid oxidase.By the function of POD,H2O2 reacts with 4AAP and HTIB, producing red material. So calculate the concentration of GA in sample by assaying the absorbance of the red material.
2. Measurement of Albumin Albumin reacts with BCG at pH4.2, producing blue-green material. Calculate the ALB concentration by assaying the absorbance.
3. Ratiocalcution(%)
Ratio(%)= (Concentration of GA/Concentration of ALB)×0.95+1.9%
- Santiago Rodriguez-Segade et al. Progression of nephropathy in type-2 diabetes: The glycation gap is a significant predictor after adjustment for glycohemoglobin (HbA1c). Clinical Chemistry, 2011, 57(2):264-271
- M. Koga, et al. Glycated albumin and glycated hemoglobin are infuenced differently by endogenous insulin secretion in patients with type 2 diabetes. Diabetescare,2010,33(2):270-272
- T. Kouzuma, et al. An enzymatic method for the measurementofglycatedalbumininbiologicalsamples. ClinicaChimicaActa,2002,324(1-2):61-71