Glutamate Oxaloacetate Transaminase Assay Kit (GOT/AST)
The Aspartate Aminotransferase (AST) assay is used for the quantitation of aspartate aminotransferase in human serum. This product is suitable for Manualuse.
Aspartate Aminotransferase (AST), also referred to as glutamate oxaloacetate transaminase (GOT), is an enzyme involved in amino acid metabolism. The activity of AST is in the serum is significantly increased during heart, liver, kidney and muscle diseases (tissue injuries, functional disorders). The activity of the enzyme is increased 4-8 hours following a myocardial infarction, reaching its peak in 2-3 days and declining on the fifth and sixth days.
Two substrates participate in the reaction catalyzed by AST, L-aspartate and Oxoglutarate. With the help of NADH coenzyme, Malate dehydrogenase (MDH) contained in the reagent catalyses the transformation of Oxalacetate released in the first reaction. The oxidoreductive process of NADH/NAD+ is indicated by a decrese in absorbance at 340 nm. The Lactate dehydrogenase (LDH) in the medium counteracts the disturbingeffect of Pyruvate contained in the sample.
AST
L-aspartate+α-ketoglutarate → Oxalacetate+L-glutamate
MDH
Oxalacetate+NADH+H+ → Mlactate+NAD++H2O
- Wroblewski F, La Due J.S: Ann Intern Med. 1956; 45:801.
- Wroblewski F, La Due J.S: Proc Soc Exp Biol Med 1956; 91:569.