Glucose Assay Kit (GLU)
For the in vitro quantitative determination of glucose in serum.
Determination of glucose concentration is important in the diagnosis and treatment disorders of carbohydrate metabolism. Increased levels of glucose are found in diabetes mellitus, hyperparathyroidism, pancreatitis, renal failure. Decreased levels are found in insulinoma, hypothyroidism, hypopituitarism and extensive liver disease.
Glucose oxidase (GOD) converts the sample glucose into gluconate. The hydrogen peroxide (H2O2) produced in the reaction is degraded by peroxidase (POD) and gives a colored product Phenol and 4-Aminoantipyrine which is measurable using Trinder indicator reaction at 505 nm. The increase in absorbance correlates with the glucose concentration of the sample.
GOD
Glucose + O2 → Gluconicacid + H2O2
POD
2H2O2+Phenol+4-Aminoantipyrine → Redquinine + 4H2O
- Barham, D, andTrinder, P. Analyst 1972; 97: 142.
- Teuscher, A. and Richterich, P. Schweiz Med. Wschr.1971; 101: 345 and 390.