Ghrelin Acylated Mouse/Rat Express ELISA
Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is necessary for the biological activity mentioned below. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.
Features
- The kit measures plasma acylated ghrelin.
- Special blood collection procedure is needed.
- Quality controls are rat-plasma based.
Research topic
Animal studies, Diabetology – Other Relevant Products, Energy metabolism and body weight regulation
Type
Sandwich ELISA, AChE-labelled antibody
Applications
Plasma-EDTA+PHMB
Sample Requirements
20 µl/well
Storage/Expiration
Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Calibration Curve
Limit of Detection
The limit of detection, calculated as the concentration of Acylated Ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.8 pg/mL. Due to the minimal plasma dilution (1:5), the limit of detection in the samples is less than 5 pg/mL.
Intra-assay (Within-Run)
CV = 8.2% (20h at +4°C) CV = 5.5% (3h at RT)
Inter-assay (Run-to-Run)
CV = 8.2% (20h at +4°C) CV = 6.5% (3h at RT)
Spiking Recovery
90.0% (20h at +4°C) 99.8% (3h at RT)
Dilutation Linearity
104.6%
– van Ginhoven TM, Huisman TM, van den Berg JW, Ijzermans JN, Delhanty PJ, de Bruin RW. Preoperative fasting induced protection against renal ischemia/reperfusion injury is independent of ghrelin in mice. Nutr Res. 2010 Dec;30 (12):865-9