Ghrelin Acylated Human Easy Sampling ELISA
Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is necessary for the biological activity mentioned below. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.
Research topic
- Diabetology – Other Relevant Products, Energy metabolism and body weight regulation
Type
Sandwich ELISA, AChE-labelled antibody
Applications
Plasma
Sample Requirements
50 µl/well
Storage/Expiration
Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Calibration Range
2.0–250 pg/ml
Limit of Detection
The Limit of Determination, calculated as the concentration of Acylated Ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 2 pg/mL. Due to the minimal plasma dilution (1:2), the limit of detection in the samples is 4 pg/mL.
– Yu CH, Chu SC, Chen PN, Hsieh YS, Kuo DY. Participation of ghrelin signalling in the reciprocal regulation of hypothalamic NPY/POMC-mediated appetite control in amphetamine-treated rats. Appetite. 2017 Jun 1;113:30-40. doi: 10.1016/j.appet.2017.02.010