Fructosamine Assay Kit (FRUC)
For the in vitro quantitative determination of fructosamine in serum.
The fructosamine are formed in blood from glucose present therein. The carbonyl group of the glucose reacts with free protein amino residues causing the formation of Schiff’s base. The half life time of the fructosamine is 17-20 days. So fructosamine determination is suitable for a long-term (1-3 weeks) monitoring of sugar metabolism for patients with diabetes, especially with type Ⅱ diabetes mellitus, and also suitable for drug efficacy monitoring.
The serum’s fructosamine is one kind of macromolecule alkone amines compounds. It can make nitrotetrazolium blue chloride reduced to formazan under the alkalinity condition. The quantity of the formazan is direct proportional to the fructosamine concentration. The color measured at 540 nm (530-550 nm), is directly propo-rtional to the fructosamine concentration.
- Howey JEA, Browning MCK, Fraser CG. Assay of serum fructosamine that minimizes standardization and matrix problems: Use to assess components of biological va-riation. Clin Chem 1987; 33: 269272.