Ep-CAM (MK-1) Human, Mouse Monoclonal Antibody, Clone: MK-1-25
The Ep-CAM antigen (Epithelial Cell Adhesion Molecule), also termed as MK-1, GA733–2, KSA, 17–1A or EGP40, is a 40 kDa type-I transmembrane glycoprotein with properties of Ca2+ -independent homotypic intracellular adhesion molecule. The full leghth of the molecule is 314 amino acids. Structurally, Ep-CAM is not related to any of the major families of adhesion molecules. The Ep-CAM antigen is overexpessed on the majority of tumor cells of most human epithelia of variety of tumor tissues such as stomach, colon, pancreas, gall bladder, bile duct, mammary gland, breast and lung carcinoma. Ep-CAM has been suggested to be involved in the differentiation and growth of epithelial cells under normal physiological conditions through its homotypic cell-cell adhesion activity. As it is overexpressed on most carcinomas, Ep-CAM antigen has been used as a target for diagnosis and therapy of cancer.
Type
Monoclonal Antibody
Applications
Western blotting, ELISA, Immunohistochemistry
Antibodies Applications
Source of Antigen
E.coli
Hosts
Mouse
Isotype
IgG1
Clone
MK-1-25
Preparation
The antibody is a mouse monoclonal antibody against recombinant Human Ep-CAM.
Species Reactivity
Human. Does not react with mouse. Not yet tested in other species.
Purification Method
Affinity chromatography on a column with immobilized protein G.
Antibody Content
0.1 mg (determined by BCA method, BSA was used as a standard)
Formulation
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody
SDS PAGE – to determine purity of the antibody
Note
This product is for research use only.
– Abe H, Kuroki M, Imakiire T, Yamauchi Y, Yamada H, Arakawa F, Kuroki M. Preparation of recombinant MK-1/Ep-CAM and establishment of an ELISA system for determining soluble MK-1/Ep-CAM levels in sera of cancer patients. J Immunol Methods. 2002 Dec 15;270 (2):227-33
– Schmetzer O, Moldenhauer G, Nicolaou A, Schlag P, Riesenberg R, Pezzutto A. Detection of circulating tumor-associated antigen depends on the domains recognized by the monoclonal antibodies used: N-terminal trimmed EpCAM-levels are much higher than untrimmed forms. Immunol Lett. 2012 Apr 30;143 (2):184-92