Dehydroepiandrosterone Sulfate ELISA
Dehydroepiandrosterone sulfate (DHEAS) is produced by the adrenals and gonads. As a result, the determination of the level of DHEA-S in serum is important in the evaluation of the functional state of these glands. DHEAS is a precursor of testosterone and estrone. Besides the adrenals in females, the ovaries have been shown to be an important source of DHEAS. It has been reported that there is a fluctuation day by day of DHEAS in women during the ovulatory cycle. An abnormal testosterone level in women should be accompanied by the estimation of serum DHEAS. The use of serum testosterone determination in conjunction with Elisa of DHEAS can be used to determine if the source of excess androgen production is ovarian or adrenal.
Research topic
Steroid hormones
Type
Competitive ELISA, Immobilized antigen
Applications
Serum
Sample Requirements
50 μl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0 .005–10 μg/ml
Limit of Detection
0.005 μg/ml
Intra-assay (Within-Run)
CV = 9.3%
Inter-assay (Run-to-Run)
CV = 9.2%
Spiking Recovery
99,50%
Dilutation Linearity
95,70%
– Stefanska A, ORCID: 0000-0002-5295-2236, Ponikowska I, Sypniewska G. A-FABP concentration is more strongly associated with cardiometabolic risk factors and the occurrence of metabolic syndrome in premenopausal than in postmenopausal middle-aged women. Biomed Res Int. 2014;2014:645762