Cytomegalovirus (CMV) IgG ELISA
The Calbiotech CMV IgG ELISA Kit is intended for the detection of IgG antibody to Cytomegalovirus (CMV) in human serum or plasma.
Cytomegalovirus (CMV) is a member of the herpes group of viruses. Most adults and children who catch CMV have no symptoms and are not harmed by the virus. CMV infection is of clinical significance primarily in pregnant women, newborn infants with possible congenital infection, immunosuppressed transplant patients and individuals with AIDS. CMV is so prevalent as over 60% of people catch the infection at some time in their lives. Significant increases in CMV IgG antibody by ELISA suggest recent infection or reactivation of a latent CMV infection. ELISA can detect CMV IgM antibody in both primary CMV infections (93-100%) and in reactivated infection (40%). An IgM response may be reduced or absent in immunocompromised patients with active infection. In transplant patients the CMV infection can be associated with higher morbidity and mortality.
Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.
- Mangano WE; Gruninger RP. Use of viral cultures and serologic tests for cytomegalovirus infection. Rational or random? Am J Clin Pathol 1996;106(2):180-4.
- Gupta CK; Leszczynski J; Gupta RK; Siber GR. An enzyme immunoassay based micro-neutralization test for titration of antibodies to human cytomegalovirus (CMV) and its correlation with direct ELISA measuring CMV IgG antibodies. Biologicals 1996;24(1):41-9.
- Guti´errez J; Maroto MC. A comparison of two ELISA methods for the investigation of anti-cytomegalovirus IgG antibodies. Microbios 1997;90(364-365):151-4.
- Gratacap-Cavallier B; Bosson JL; Morand P; Dutertre N; Chanzy B; Jouk PS; Vandekerckhove C; Cart-Lamy P; Seigneurin JM. Cytomegalovirus seroprevalence in French pregnant women: parity and place of birth as major predictive factors. Eur J Epidemiol 1998;14(2):147-52.
- Schmidt CA; Oettle H; Peng R; Neuhaus P; Blumhardt G; Lohmann R; Wilborn F; Osthoff K; Oertel J; Timm H; et al. Comparison of polymerase chain reaction from plasma and buffy coat with antigen detection and occurrence of immunoglobulin M for the demonstration of cytomegalovirus infection after liver transplantation. Transplantation 1995;59(8):1133-8.
- Rosenthal SL; Stanberry LR; Biro FM; Slaoui M; Francotte M; Koutsoukos M; Hayes M; Bernstein DI. Seroprevalence of herpes simplex virus types 1 and 2 and cytomegalovirus in adolescents. Clin Infect Dis 1997;24(2):135-9.