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Cystatin C Canine ELISA

Regulatery Status: RUO
Type: Sandwich ELISA, HRP-labelled antibody
Other Names Status: Post G-globulin, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3
Species: Canine
Catalog No Size
Product Catalog No: RD491009100R Pack Size: 96 wells(1 kit)

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Product Features

Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36 kDa and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer’s di­sease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.

Features

  • It is intended for research use only
  • The total assay time is less than 3 hours
  • The kit measures total cystatin C in canine serum and urine
  • Assay format is 96 wells
  • Quality Controls are animal serum based. No human sera are used
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Animal studies, Neural tissue markers, Renal disease

Techical Sheet / Info

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Urine

Sample Requirements

5 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.31–10 ng/ml

Limit of Detection

0.005 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 6.3%

Inter-assay (Run-to-Run)

n = 5; CV = 5.7%

Spiking Recovery

103,70%

Dilutation Linearity

91,40%

Crossreactivity

bovine  Non-detectable
cat  Non-detectable
 goat  Non-detectable
hamster  Non-detectable
horse  Non-detectable
dog  Yes
monkey  Non-detectable
mouse  Non-detectable
pig  Non-detectable
rabbit  Non-detectable
rat  Non-detectable
sheep  Non-detectable
human  Non-detectable
chicken  Not tested
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References

– Martineau AS, Leray V, Lepoudere A, Blanchard G, Bensalem J, Gaudout D, Ouguerram K, Nguyen P, ORCID: http://orcid.org/0000-0002-4263-025X. A mixed grape and blueberry extract is safe for dogs to consume. BMC Vet Res. 2016;12 (1):162

– Tvarijonaviciute A, Ceron JJ, Holden SL, Biourge V, Morris PJ, German AJ. Effect of weight loss in obese dogs on indicators of renal function or disease. J Vet Intern Med. 2013 Jan-Feb;27 (1):31-8

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