Coxsakie B Virus IgM

Microplates are coated with native Coxsackie virus B antigens derived from tissue culture, containing its major subtypes. In the 1st incubation, the solid phase is treated with diluted samples and anti-CoxB IgM are captured, if present, by the antigens. After washing out all the other components of the sample, in the 2nd incubation bound anti-CoxB IgM are detected by the addition of anti hIgM antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-CoxB IgM antibodies present in the sample. The presence of IgM in the sample may therefore be determined by means of a cut-off value able to discriminate between low and high samples. Neutralization of IgG anti-CoxB, carried out directly in the well, is performed in the assay in order to block interferences due to this class of antibodies in the determination of IgM.