CoxB IgM
Enzyme Immuno Assay (ELISA) for the determination of IgM antibodies to Coxsackievirus type B (CoxB) in human plasma and sera.
Coxsackievirus belongs to a group of viruses called enteroviruses in particular they are Picornaviruses.They are present in two main groups, A and B. Most Coxsackievirus infections are not serious. They typically cause only mild signs and symptoms, such as fever, rash, sore throat, joint pain and headache. Symptoms usually last about a week. Coxsackievirus infection occurs most often in young children. Group A viruses are associated with aseptic meningitis, colds, acute hemorrhagic conjunctivitis and acute myocardiopathies and group B are associated with acute myocarditis and a polio-like paralysis. Syndromes associated particularly with Coxsackie B virus are pleurodynia, also known as Bornholm disease or devil’s grippe, which presents with severe pleuritic chest pain, sometimes accompanied by abdominal pain and vomiting, asceptic meningitis, colds, and myocardial or pericardial infections. Recently immunochemical techniques have been applied to the early diagnosis of CoxB infection with ELISA tests for IgM, as a serological marker of recent infection.
Microplates are coated with native Coxsackievirus B antigens derived from tissue culture, containing its major subtypes. In the 1st incubation, the solid phase is treated with diluted samples and anti-CoxB IgM are captured, if present, by the antigens. After washing out all the other components of the sample, in the 2nd incubation bound anti-CoxB IgM are detected by the addition of anti hIgM antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-CoxB IgM antibodies present in the sample. The presence of IgM in the sample may therefore be determined by means of a cut-off value able to discriminate between negative and positive samples. Neutralization of IgG anti-CoxB, carried out directly in the well, is performed in the assay in order to block interferences due to this class of antibodies in the determination of IgM.
- King ML, Shaikh A, Bidwell D, Voller A, Banatvala JE. Lancet. 1983 Jun 25;1(8339):1397-9. PMID: 6134178
- Banatvala JE, Bryant J, Schernthaner G, Borkenstein M, Schober E, Brown D, De Silva LM, Menser MA, Silink M. Lancet. 1985 Jun 22;1(8443):1409-12. PMID: 2861361
- El-Hagrassy MM, Banatvala JE, Coltart DJ. Lancet. 1980 Nov 29;2(8205):1160-2. PMID: 6107769
- Frisk G, Fohlman J, Kobbah M, Ewald U, Tuvemo T, Diderholm H, Friman G. J Med Virol. 1985 Nov;17(3):219-27. PMID: 2999322
- Schernthaner G, Banatvala JE, Scherbaum W, Bryant J, Borkenstein M, Schober E, Mayr WR. Lancet. 1985 Sep 21;2(8456):630-2. PMID: 2863632
- Pugh SF. J Clin Pathol. 1984 Apr;37(4):433-9. PMID: 6323548
- Friman G, Fohlman J, Frisk G, Diderholm H, Ewald U, Kobbah M, Tuvemo T. Acta Paediatr Scand Suppl. 1985;320:14-9. PMID: 3010631