CoxB IgG
Enzyme Immuno Assay (ELISA) for the determination of IgG antibodies to Coxsackievirus type B (CoxB) in human plasma and sera.
Coxsackievirus belongs to a group of viruses called enteroviruses in particular they are Picornaviruses.They are present in two main groups, A and B. Most Coxsackievirus infections are not serious. They typically cause only mild signs and symptoms, such as fever, rash, sore throat, joint pain and headache. Symptoms usually last about a week. Coxsackievirus infection occurs most often in young children. Group A viruses are associated with aseptic meningitis, colds, acute hemorrhagic conjunctivitis and acute myocardiopathies and group B are associated with acute myocarditis and a polio-like paralysis. Syndromes associated particularly with Coxsackie B virus are pleurodynia, also known as Bornholm disease or devil’s grippe, which presents with severe pleuritic chest pain, sometimes accompanied by abdominal pain and vomiting, asceptic meningitis, colds, and myocardial or pericardial infections. Recently immunochemical techniques have been applied to the early diagnosis of CoxB infection with ELISA tests for IgG, as a serological marker of past infection.
Microplates are coated with native Coxsackievirus B antigens derived from tissue culture, containing its major subtypes. In the 1st incubation, the solid phase is treated with diluted samples and anti-CoxB IgG are captured, if present, by the antigens. After washing out all the other components of the sample, in the 2nd incubation bound anti-CoxB IgG are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-CoxB IgG antibodies present in the sample. The presence of IgG in the sample may therefore be determined by means of a cut-off value able to discriminate between negative and positive samples.
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