Cortisol Salivary HS
An enzyme immunoassay for measurement of active free cortisol (hydrocortisone and hydroxycorticosterone) in saliva.
The Salivary Cortisol HS ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal rabbit antibody directed towards an antigenic site on the cortisol molecule. Endogenous cortisol of a donor sample competes with a cortisol-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of cortisol in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of cortisol in the donor sample.
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