Cornifin-B Human, Sheep Polyclonal Antibody
Cornifin-B occurs in keratinocytes. It is cross-linked to membrane proteins by transglutaminase which leads to formation of an insoluble envelope. It is expressed during differentiation in squamous tissues such as epidermis, esophagus, tongue or trachea.
Type
Polyclonal Antibody
Applications
Immunohistochemistry
Source of Antigen
E. coli
Hosts
Sheep
Preparation
The antibody was raised in sheep by immunization with the recombinant Human Cornifin-B.
Amino Acid Sequence
The immunization antigen (10.9 kDa, calculated) is a protein containing 97 AA of recombinant Human Cornifin-B. N-terminal His-tag, 9 extra AA (highlighted).
Species Reactivity
Human
Purification Method
Immunoaffinity chromatography on a column with immobilized recombinant Human Cornifin-B.
Antibody Content
0.1 mg (determined by BCA method, BSA was used as a standard)
Formulation
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Reconstitution
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody SDS PAGE – to determine purity of the antibody