Complement C3 Assay Kit (C3)
The Complement C3 (C3) assay kit is used for the quantitation of complement C3 in human serum or plasma.
Product Features
Complement components are synthesised primarily in the liver. C3 and C4 are the components most frequently measured. C3 and C4 are increased in a number after an Acute Phase Response but they are weak and late reacting. Acute Phase Protein C3 and C4 are also elevated in biliary obstruction.
Assay Principle
This assay is based on the reaction between antigen and antibody. This reaction forms an insoluble complex producing a turbidity, which is measured spectrophotometrically. The amount of complex formed is directly proportional to the amount of C3 in the sample.
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References
- Karl J, Engel WD. Determination of Apolipoprotein A1 and B without sample dilution. Poster presented at the 57th meeting of the European Atherosclerosis Society, Lisbon and the IX European Congress of Clinical Chemistry, Cracow 1991.
- Burtis CA, Ashwood ER. Tietz Fund. Of Clin. Chem. 5th ed. 30-54, 335-336, 462-494 and 972973.
- Consensus values of the Deutsche Gesellschaft fur Laboratoriums-medizin, the Deutsche Gesellschaft fur Klinische Chemie and the Verband der Diagnostica-Industrie.V. (VDGH). DG KlinischeChemie Mitteilungen 1995; 26:119-122.
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