Club Cell Protein (CC16) Human ELISA
Human Club cell protein (CC16, CC10, uteroglobin, urinary protein 1 or Clara cell secretory protein) is a member of the secretoglobin family of proteins and is a secreted product of nonciliated
bronchiolar Club cells. Its function remains to be fully elucidated but there is convincing data suggesting its role as an immune-modulating and anti-inflammatory agent. Club cell protein inhibits phospholipase A2 activity as well as interferon gamma signaling and Th1 vs. Th2 lymphocyte regulation.
Club cell protein concentrations have been determined in serum, plasma and bronchoalveolar lavage fluid in numerous studies since 1994. In serum, its increase is associated with age and asbestos, nitrogen chloride and ozone exposure. Higher levels of CC16 were demonstrated in patients with sarcoidosis, pulmonary fibrosis and high PEEP ventilation. Decreased serum
CC16 levels are found after pulmonary resection in smokers and in subjects with chronic obstructive pulmonary disease, asthma or silica exposure.
Decreased CC16 concentrations were also found in the amniotic fluid of fetuses suffering from pulmonary hypoplasia caused by various mechanisms (diaphragmatic hernia, diabetic fetopathy, Turner and Down syndrome). In pleural effusions, the CC16 concentration appears to be associated with its diffusion from the lung as evidenced by high CC16 levels in cardiac pleural congestion.
Based on the above reports Club cell protein might be perspective useful diagnostic marker of pulmonary diseases and injuries.
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate
Sample Requirements
5 µl/well
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
1.57–50 ng/ml
Limit of Detection
46 pg/ml
Intra-assay (Within-Run)
n = 8; CV = 3.4%
Inter-assay (Run-to-Run)
n = 4; CV = 4.7%
Spiking Recovery
96,00%
Dilutation Linearity
103,60%
Crossreactivity
bovine Non-detectable
cat Non-detectable
dog Non-detectable
goat Non-detectable
hamster Non-detectable
horse Non-detectable
pig Non-detectable
rabbit Non-detectable
rat Non-detectable
sheep Non-detectable
chicken Not tested
monkey Yes
mouse Yes
human Yes
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