Cholyglycine Assay Kit (CG)
For the quantitative in vitro determination of Cholyglycine (CG) in human serum.
Serum Cholyglycine (Cholyglycine, CG) is one of the binding cholic acid combined by cholic acid and glycine, within the liver cells, cholesterol transformed into the primary bile acid through the extremely complex enzymatic reaction. Including cholic acid (CA) and chenodeoxycholic acid (CD – CA). there are three hydroxyl (C3, C7, C12) in the steroid nucleus of cholic acid, the hydroxyl at the end of side chains using peptide bonds with glycine, the molecular weight is 462 u [1].
Under normal circumstances, the cholic acid content in the peripheral blood is very little, no matter on an empty stomach or after eating, the concentration of serum CG is stable at a low level of a normal adult. When the liver cell is damaged, liver cells’ ability of absorbing CG will drop, cause the increase of the CG content in the serum; When the bile stasis, the liver’s excretion of bile acid occurs disorder, and the CG content in the reflux circulated blood is increse, also makes the blood CG content increased [1].
Cholestasis syndrome during pregnancy (ICP), at present, the recognized index of ICP is CG values, which sensitivity is much higher than that of TBA, so at present’s clinic, the TBA can not replace CG at all.
CG and super sensitized CG antibody latex particle reagent react and appear agglutination, test the absorbance at the wavelength of 600 nm, the changing degree is proportional to the CG content of the sample.
- Dehui Wan, Duniju Liu. The clinical analysis of the serum liver serum chileglycine levels in chronic liver disease patients, The practical clinical medicine, 2012, 13 (4) : 5-6.
- Xiao-ling Hu, Wei-guang Wang, Wei Wu. Pregnant women. The clinical significance of testing the serum acid and chileglycine, Radiation immunology journal. 2004 (4) : 294-295.