Chlamydia Trachomatis IgM
Enzyme ImmunoAssay (ELISA) for the determination of IgM antibodies to Chlamydia Trachomatisin human plasma and sera.
Chlamydia trachomatis is a bacterium-like obligate intracellular organism that counts at least 15 recognized serotypes. C.trachomatis is one of the three distinct species within the genus Chlamydia (trachomatis, psittaci and pnemoniae).
C.trachomatis infection in adults is responsible of most of sexually acquired urethritis in men, mucopurulent cervicitis in women, pelvic inflammatory disease, lymphogranuloma venereum, most of acute urethral syndromes, ocular infections, proctocolitis and epididymitis. In infants, the organism is responsible of pneumonia and conjunctivitis.
Infections due to C.trachomatis stimulates the patient to generate a strong immunological response both in IgG, lasting a long time, and IgA and IgM, whose presence is more correlated with an ongoing infection or a recent event.
The determination of species-specific IgG, IgM and IgA is a helpful tool for the clinician to identify the infective agent and to decide the right therapy.
Microplates are coated with a species-specific polypeptide derived from C.trachomatis major outer membrane antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-CT IgM are captured, if present, by the antigens.
After washing out all the other components of the sample, in the 2nd incubation bound anti-CT IgM are detected by the addition of anti hIgM antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-CT IgM antibodies present in the sample.
The presence of IgM in the sample may therefore be determined by means of a cut-off value able to discriminate between negative and positive samples.
Neutralization of IgG anti-CT, carried out directly in the well, is performed in the assay in order to block interferences due to this class of antibodies in the determination of IgM.
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