Chlamydia trachomatis IgG
Enzyme ImmunoAssay (ELISA) for the quantitative determination of IgG antibodies specific to Chlamydia trachomatis in human plasma and sera.
Chlamydia trachomatis is a bacterium-like obligate intracellular organism that counts at least 15 recognized serotypes. C.trachomatis is one of the three distinct species within the genus Chlamydia (trachomatis, psittaci and pnemoniae).
C.trachomatis infection in adults is responsible of most of sexually acquired urethritis in men, mucopurulent cervicitis in women, pelvic inflammatory disease, lymphogranuloma venereum, most of acute urethral syndromes, ocular infections, proctocolitis and epididymitis. In infants, the organism is responsible of pneumonia and conjunctivitis.
Infections due to C.trachomatis stimulates the patient to generate a strong immunological response both in IgG, lasting a long time, and IgA, whose presence is more correlated with an ongoing infection or a recent event.
The determination of species-specific IgG, IgA and IgM is a helpful tool for the clinician to identify the infective agent and to decide the right therapy.
Microplates are coated with an immunodominant speciesspecific polypeptide derived from Chlamydia trachomatis major outer-membrane antigen (MOMP), that makes the assay very specific for C.trachomatis (no cross reaction with C.pneumoniae).
In the 1st incubation, the solid phase is treated with diluted samples and anti-C.trachomatis IgG are captured, if present, by the solid phase.
After washing out all the other components of the sample, in the 2nd incubation bound anti-C.trachomatis IgG are detected by the addition of anti hIgG antibody, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-C.trachomatis IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (Uarb/ml) as no international standard is available.
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