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Chlamydia trachomatis IgA

Enzyme ImmunoAssay (ELISA) for the qualitative determination of IgA antibodies to Chlamydia Trachomatis in human plasma and sera. The product is intended for the follow-up of patients showing pathologies referable to Chl. Trachomatis infection.

Regulatery Status: CE
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Product Catalog No: CTA Pack Size: 96 Tests

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Summary

Chlamydia trachomatis is a bacterium-like obligate intracellular organism that counts at least 15 recognized serotypes. C.trachomatis is one of the three distinct species within the genus Chlamydia (trachomatis, psittaci and pneumoniae).

C.trachomatis infection in adults is responsible of most of sexually acquired urethritis in men, mucopurulent cervicitis in women, pelvic inflammatory disease, lymphogranuloma venereum, most of acute urethral syndromes, ocular infections, proctocolitis and epididymitis. In infants, the organism is responsible of pneumonia and conjunctivitis.

Infections due to C.trachomatis stimulates the patient to generate a strong immunological response both in IgG, lasting a long time, and IgA, IgM whose presence is more correlated with an ongoing infection or a recent event.

The determination of species-specific IgG, IgM and IgA is a helpful tool for the clinician to identify the infective agent and to decide the right therapy.

Test Principle

Microplates are coated with a species-specific polypeptide derived from C.trachomatis major outer membrane antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-C.trachomatis IgA are captured, if present, by the antigens.

After washing out all the other components of the sample, in the 2nd incubation bound anti-C.trachomatis IgA are detected by the addition of anti hIgA antibody, labelled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-C.trachomatis IgA antibodies present in the sample.

IgA in the sample are then determined by a cut-off value able to discriminate between the negative and the positive population. Interferences due to IgG are blocked by means of a Neutralizing Reagent directly added to the sample in the well.

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References
  • Bas S, Genevay S, Schenkel MC, Vischer TL.Importance of speciesspecific antigens in the serodiagnosis of Chlamydia trachomatis reactive arthritis.Rheumatology (Oxford). 2002 Sep;41(9):1017-20.PMID: 12209035 [PubMed – indexed for MEDLINE]
  • Marston EL, James AV, Parker JT, Hart JC, Brown TM, Messmer TO, Jue DL, Black CM, Carlone GM, Ades EW, Sampson J.Newly characterized species-specific immunogenic Chlamydophila pneumoniae peptide reactive with murine monoclonal and human serum antibodies.Clin Diagn Lab Immunol. 2002 Mar;9(2):446-52.PMID: 11874892 [PubMed – indexed for MEDLINE]
  • Morré SA, Munk C, Persson K, Krüger-Kjaer S, van Dijk R, Meijer CJ, van Den Brule AJ.Comparison of three commercially available peptide-based immunoglobulin G (IgG) and IgA assays to microimmunofluorescence assay for detection of Chlamydia trachomatis antibodies.J Clin Microbiol. 2002 Feb;40(2):584-7.PMID: 11825974 [PubMed – indexed for MEDLINE]
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  • Klein M, Kötz A, Bernardo K, Krönke M.Detection of Chlamydia pneumoniae-specific antibodies binding to the VD2 and VD3 regions of the major outer membrane protein.J Clin Microbiol. 2003 May;41(5):1957- 62.PMID: 12734234 [PubMed – indexed for MEDLINE]
  • Mygind P, Christiansen G, Persson K, Birkelund S.Detection of Chlamydia trachomatis-specific antibodies in human sera by recombinant major outer-membrane protein polyantigens.J Med Microbiol. 2000 May;49(5):457-65. PMID: 10798559 [PubMed – indexed for MEDLINE]
  • Jones CS, Maple PA, Andrews NJ, Paul ID, Caul EO.Measurement of IgG antibodies to Chlamydia trachomatis by commercial enzyme immunoassays and immunofluorescence in sera from pregnant women and patients with infertility, pelvic inflammatory disease, ectopic pregnancy, and laboratory diagnosed Chlamydia psittaci/Chlamydia pneumoniae infection.J Clin Pathol. 2003 Mar;56(3):225-9.PMID: 12610104 [PubMed – indexed for MEDLINE]
  • Portig I, Goodall JC, Bailey RL, Gaston JS.Characterization of the humoral immune response to Chlamydia outer membrane protein 2 in chlamydial infection.Clin Diagn Lab Immunol. 2003 Jan;10(1):103-7.PMID: 12522047 [PubMed – indexed for MEDLINE]
  • Bas S, Vischer TL.Chlamydia trachomatis antibody detection and diagnosis of reactive arthritis.Br J Rheumatol. 1998 Oct;37(10):1054- 9.PMID: 9825743 [PubMed – indexed for MEDLINE]
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