Chlamydia Pneumoniae IgM
Enzyme ImmunoAssay (ELISA) for the determination of IgM antibodies to Chlamydia Pneumoniae in human plasma and sera. The product is intended for the follow-up of patients showing respiratory pathologies referable to Chl. pneumoniae infection.
Chlamydia pneumoniae, like all the Chlamydia, is an obligate intracellular bacterium, which stains gram-negative. The organism shares about 10% DNA sequence homology with C.trachomatis and C.psittaci.
Transmission of infection occurs person-to-person.
Most of adults are seropositive as the organism is quite frequent in all the world.
Clinical syndromes due to C.pneumoniae infection are atypical pneumonia, bronchitis, pharyngitis and sinusitis. Diseases are usually mild to moderate in severity, but symptoms may be prolonged.
Both IgG and IgA classes of antibodies are generated upon infection in the patient. While IgG antibodies tends to last for years, the presence of IgA is more correlated with an ongoing infection or with a recent event.
The determination of species-specific antibodies may be an useful tool for the clinician in the identification of the infecting organism and in the definition of the right therapy.
Microplates are coated with a preparation of native C.pneumoniae. In the 1st incubation, the solid phase is treated with diluted samples and anti-C.pneumoniae IgM are captured, if present, by the solid phase.
After washing out all the other components of the sample, in the 2nd incubation bound anti-CP IgM are detected by the addition of anti hIgM antibody, labeled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-CP IgM antibodies present in the sample.
The presence of IgM in the sample may therefore be determined by means of a cut-off value able to discriminate between negative and positive samples.
Neutralization of IgG anti-CP, carried out directly in the well, is performed in the assay in order to block interferences due to this class of antibodies in the determination of IgM.
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