CHIK IgG/IgM 40T
TruQuick CHIK IgG/IgM is a rapid immunoassay for the qualitative detection of IgG and IgM antibodies to Chikungunya in whole blood, serum or plasma.
Chikungunya is a rare viral infection transmitted by the bite of infected mosquitoes, predominantly Aedes aegypti mosquito. It is characterized by a rash, fever, and severe joint pain (arthralgia) that usually lasts for three to seven days. The name is derived from the Makonde word meaning ‘that which bends up’ in reference to the stooped posture developed as a result of the arthritic symptoms of the disease. It occurs during the rainy season in tropical areas of the world, primarily in Africa, South-East Asia, southern India and Pakistan.
The symptoms are most often clinically indistinguishable from those observed in dengue fever. Indeed, dual infection of dengue and chikungunya has been reported in India.3 Unlike dengue, hemorrhagic manifestations are relatively rare and most often the disease is a self limiting febrile illness. Therefore it is very important to clinically distinguish dengue from Chikungunya infection.
Chikungunya is diagnosed based on serological analysis and viral isolation in mice or tissue culture. An IgM immunoassay is the most practical lab test method.
- Bring the pouch to room temperature before opening it. Remove the Test Cassette from the sealed pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
- Place the cassette on a clean and level surface.
- For Serum or Plasma specimen: Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 40 μL) to the specimen area, then add 2 drops of Buffer (approximately 80 μL) and start the timer. See illustration below.
- For Venipuncture Whole Blood specimen: Hold the dropper vertically and transfer 1 drop of whole blood (approximately 40 μL) to the specimen area, then add 2 drops of Buffer (approximately 80 μL) and start the timer. See illustration below.
- For Fingerstick Whole Blood specimen:
- To use a capillary tube: Fill the capillary tube and transfer approximately 40 μL of fingerstick whole blood specimen to the specimen area of Test Cassette, then add 2 drops of Buffer (approximately 80 μL) and start the timer. See illustration below.
- To use hanging drops: Allow 1 hanging drop of fingerstick whole blood specimen (approximately 40 μL) to fall into the specimen area of Test Cassette, then add 2 drops of Buffer (approximately 80 μL) and start the timer. See illustration below.
- Wait for the colored line(s) to appear. Read the result at 15 minutes, do not interpret the result after 20 minutes.
- Shah KV, Gibbs CJ Jr, Banerjee G. Virological investigation of the epidemic of haemorrhagic fever in Calcutta: isolation of three strains of Chikungunya virus. Indian J Med Res. 1964;52 676-83.
- Powers AM, Brault AC, Tesh RB, Weaver SC. Re-emergence of Chikungunya and O’nyongnyong viruses: evidence for distinct geographical lineages and distant evolutionary relationships. J Gen Virol. 2000;81:471-9.
- Myers RM, Carey DE. Concurrent isolation from patient of two arboviruses, Chikungunya and dengue type 2. Science. 1967;157:1307-8.
- Thein S, La Linn M, Aaskov J, Aung MM, Aye M, Zaw A, Myint A. Development of a simple indirect enzyme-linked immunosorbent assay for the detection of immunoglobulin M antibody in serum from patients following an outbreak of chikungunya virus infection in Yangon, Myanmar. Trans R Soc Trop Med Hyg. 1992 86:438-42.
- Yamamoto K, Hashimoto K, Simizu B, Ogata T. Structural proteins of Chikungunya virus. J Virol. 1984 Jul;51(1):254-8.