Cardiac TroponinI Assay Kit (cTnI)
For the in vitro quantitative determination of cTnI in serum or plasmasamples.
Cardiac troponin consists of three subunits: cTnT, cTnI and cTnC. cTnI, with Molecular weight 21kD is regarded as a high specificity and sensitivity marker of myocardial. cTnI is a unique component of myocardial cells. Blood levels of cTnI in myocardial infarction occurs 4-6 hours and reach a peak in 12-24 hours. Troponin I in the blood has a long duration, the positive results of sustainable to 7-10 days after myocardial infarction. Now cTnI has been more and more used in myocardial ischemic injury such as acute coronary syndrome and other clinical diagnosis, risk degree and prognosis assessment, the area of myocardial infarction predict myocardial infarction thrombolytic efficacy, heart damage, congestive heart failure, severe sepsis caused by left heart failure clinical diagnosis et al. In addition, cTnI on retrospective diagnosis is significant, especially for those visiting time late, myocardial enzyme has returned to normal in patients with myocardial infarction.
An antigen-antibody reaction occurs between cTnI in a sample and anti-cTnI antibody which has been coated to latex particles. This resulting agglutination is detected as an absorbance change (500 nm), with the magnitude of the change being proportional to the quantity of cTnI in the sample. The actual concentration is then determined by interpolation from a calibration curve prepared from calibrators of known concentration.
- Bodor, S.G., et al., Development of monoclonal antibody for an assay of cardiac troponin I and preliminary results in suspected cases ofmyocardial infarction. Clin.Chem. 38:2203 (1992)
- Adams, J.E., et at, Biochemical markers of myocardial injury. Is MB creatin kinase the choice for the 1990’s. Circulation 88:750 (1993)