Carbon Dioxide (CO2) Assay
Carbon Dioxide Enzymatic Assay Kit is intended for the quantitative determination of carbon dioxide (CO2) in serum and plasma
CO2 Enzymatic Assay is a single vial liquid stable reagent system which has a neutral pH, minimizing the effect of CO2 absorption from the air. In addition to a highly stable NADH analog as a co-factor the reagent maintains integrity by rebuilding key reagent components as required for optimal performance. The assay has a linearity of 1.12 – 50 mmol/L, correlation coefficient value of 0.99, slope of 1.0447, y intercept of -0.9742 and is highly precise with Intra and Inter Assay Precision %CV’s of <3.5%. Packaged for optimal operator convenience, reagent transfer can be eliminated for most chemistry systems with instrument specific packaging options including RocheTM Hitachi 917 series, Beckman AU (400/600/640/680), Beckman Synchron CX, LX and DXC.
Carbon Dioxide Enzymatic Assay is based on two coupled enzyme reactions including phosphoenolpyruvate carboxylase (PEPC) and malate dehydrogenase (MDH). PEPC catalyzes the first reaction which produces oxaloacetate. In the presence of MDH, the reduced cofactor is oxidized by oxaloacetate. This results in a decrease of absorbance at 405 or 415 nm that is directly proportional to CO2 concentration in the sample.
