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Calcium Assay Kit (Ca)

For the in vitro quantitative determination of Calcium in serum.

Product Catalog Number Packaging Method/Format
Product Catalog No: Kit (300 Ml) Pack Size: GB400E Pack Size: R1: 6 × 50 ml Pack Size: Arsenazo III
Product Catalog No: Kit (300 Ml) Pack Size: GS401E Pack Size: R1: 6 × 50 ml
Product Catalog No: Kit (300 Ml) Pack Size: GH401E Pack Size: R1: 6 × 50 ml
Product Catalog No: Kit (100 Ml) Pack Size: GX401E Pack Size: R1: 1 × 100 ml
Product Catalog No: Kit (300 Ml) Pack Size: GT401E Pack Size: R1: 6 × 50 ml
Product Catalog No: Kit (154.8) Pack Size: GD401E Pack Size: R1: 36 × 4.3 ml
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Product Features

Calcium is the fifth most common element. Calcium exists in three physical chemical states: (1) free or ionized calcium; (2) calcium complexed to anions, including bicarbonate, lactate, phosphate, and citrate; (3) calcium bound to plasma proteins. Calcium has key roles in muscle contraction, hormone secretion, glycogen metabolism, and cell division. Increased calcium levels in serum are reported in hyperparathyroidism, cancer, metastatic bone lesions and hypervitaminosis, while decreased levels are observed in hypoparathyroidism, nephrosis, rickets, nephritis and calcium-losing syndromes.

Assay Principle

Arsenazo III reacts with calcium in solution to form a blue-purple complex. The color developed has a maximum absorbance at 650 nm and is proportional to the calcium concentration in the sample.

Arsenazo III + Ca2+ → blue-purple

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References
  • Tietz, N.W., Fundamentals of Clinical Chemistry 2nd. Edition W.B. Saunders Co., Philadelphia (1976)
  • V. Michaylova and P. Ilkova, Photometric determination of micro amounts of calcium with arsenazo III, (1971)Anal. Chim. Acta. 53:194-198
  • Barnett, R.N., et al. Performance of “kits” used for clinical chemical analysis of calcium in serum. (1973) Amer. J. Clin. Path. 59: 836-845.
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