C-Reactive Protein
This product is used on NEPHSTAR® protein analysis system for quantitative determination of human C-Reactive Protein (CRP) in serum
CRP is synthesized in liver and consists of five identical polypeptide chains forming a five-membered ring of molecular weight of 120 kD. CRP is one of the most characteristic acute-phase proteins and is considered as a reliable indicator of disease activity in various clinical conditions. Its concentration in blood increases rapidly by as much as 1000-fold upon exposure to various inflammatory stimuli. CRP has been used successfully for clinical diagnosis and monitoring of a variety of infections and diseases, including infections caused by bacteria, fungi, and viruses ; intercurrent infections in leukemia and systemic lupus erythematosus; noninfectious inflammatory diseases such as rheumatoid arthritis; and diseases with cellular necrosis such as myocardial infarction. Measurements of CRP are especially useful in distinguishing viral from bacterial infections. Among the methods used to measure CRP in serum are radial immunodiffusion, turbidimetry, nephelometry, enzyme immunoassays, etc.. The acute nature of many diseases in which CRP is relevant for diagnosis and monitoring requires a rapid, easily interpreted, quantitative test, which comes true with this kit.
Particle-enhanced immunonephelometry is applied. This method involves measuring the light scattered by insoluble complexes formed by reaction between specific protein in samples and its respective antibody covalently coupled to latex particles, and the amount of scattered light is directly proportional to the concentration of the protein under condition that antiserum is in excess. The latex particles increase the size of complexes formed and thus the amount of light as well as the test sensitivity. Concentrations are automatically calculated by reference to a calibration curve stored in the instrument.
1. Urdal P, Borch SM, Landaas S, Krutnes MB, Gogstad GO, Hjortdahl P. Rapid immunometric measurement of C-reactive protein in whole blood. Clin Chem 1992;38:580-584.
2. Chambers RE, Whicker JT, Dieppe PA. Acute phase proteins in inflammatory disease. Clin Diagnosis Lab 1988;1:29-37.
3. Morley JJ, Kushner I. Serum C-reactive protein levels in disease. Ann N Y Acad Sci 1982;389:406-18.
4. Lindback S, Heligren U, Julander I, Hanseon LO. The value of C-reactive protein as a marker of bacterial infection in patients with septicaemia, endocarditis and influenza. Scand J Infect Dis 1989;21:543-.9.