Borrelia 14kD and OspC IgM ELISA
Enzyme immunoassay for measurement of IgM antibodies against the 14 kDa and OspC antigens of Borrelia burgdorferi in human serum, plasma and CSF.
Test Principle
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The wells are coated with antigen. Specific antibodies of the sample binding to the antigen coated wells are detected by a secondary enzyme conjugated antibody (E-Ab) specific for human IgM. After the substrate reaction the intensity of the color developed is proportional to the amount of IgM-specific antibodies detected. Results of samples can be determined directly using the standard curve or calibrator.
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References
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