Apolipoprotein B
This product is used on NEPHSTAR® protein analysis system for quantitative determination of human Apolipoproterin B (ApoB) in serum
ApoB is an important component of LDL, IDL, VLDL and chylomicron. ApoB exists in two types: one is ApoB-100 in VDL/LDL; the other is ApoB-48. ApoB-100 generates in liver and ApoB-48 generates in the small intestine. ApoB is very important for transportation of cholesterol and triglyceride. Elevated levels of ApoB are frequently found in atherosclerotic vascular changes and are a risk factor for atherosclerosis.
Immunonephelometry is applied. This method involves measuring the light scattered by insoluble complexes formed by reaction between specific protein in samples and its respective antiserum, and the amount of scattered light is directly proportional to the concentration of the protein under condition that antiserum is in excess. Concentrations are automatically calculated by reference to a calibration curve stored in the instrument.
1. Bablok W et al. General Regression Procedure for Method Transformation. J Clin Chem Biochem 1988; 26:783-790
2. Naito, H.K., J. Clin. Immunoassay, 9, 155 (1986)