Apolipoprotein A1
This product is used on NEPHSTAR® protein analysis system for quantitative determination of human Apolipoprotein A1 (ApoA1) in serum
ApoAI is primarily found in high density lipoprotein (HDL) particle. It serves the function of preventing the accumulation of cholesterol loaded macrophages which deposit on the arterial wall as foam cells. This is the prominent early feature of atherosclerotic lesion formation ultimately resulting in atherosclerosis. ApoAI is a single polypeptide with a molecular weight of 28 Kd. Its primary function is to activate LCAT(lecithin cholesterol acyltransferase) within the HDL complex, which catalyzes the esterification of cholesterol. Persons with atherosclerotic vascular changes frequently exhibit decreased levels of Apo A1. Even if the concentrations of apolipoprotein B are normal, a decreased Apo A1 level may be a risk factor for atherosclerotic processes. Decreased levels of Apo A1 also occur in dyslipoproteinemias, etc..
Immunonephelometry is applied. This method involves measuring the light scattered by insoluble complexes formed by reaction between specific protein in samples and its respective antiserum, and the amount of scattered light is directly proportional to the concentration of the protein under condition that antiserum is in excess. Concentrations are automatically calculated by reference to a calibration curve stored in the instrument.
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2. Rifai, N., Ann. Clin. Lab. Science. 18, 429 (1988)