Apo B-48 Human ELISA
Plasma lipoproteins are complex substances composed of certain ratios of lipids and proteins, and are important factors for serum lipids transportation. On the surface of the lipoprotein structure, apolipoproteins are present and playing roles in stabilization of lipoprotein structure, in activation of enzymes relating to lipoprotein metabolism, and in binding with lipoprotein receptors on the cell surface. Apolipoprotein-B48 (ApoB-48) has 48% amino acid sequence of apolipoprotein B-100 which is present in lipoproteins ,VLDL, LDL and HDL of liver origin. ApoB-48 is an apolipoprotein specific to a lipoprotein, chylomicron (CM) which is formed in intestine and carries exogenous lipids derived from foods to the liver and peripheral tissues. Measurement of ApoB-48 is useful in observation and pursuit of postprandial dynamics of lipoprotein, lipid-soluble nutrients and drugs.
Features
- This is intended for research use only.
- Rapid assay (total reaction time: 2 h 50min.).
- This kit is for apolipoprotein B-48 in human serum or plasma (citric acid for plasma is not suitable).
- A small sample volume of serum or plasma (5 µl) is needed.
- Assay format is 96 wells.
- Standard Apo B-48 is derived from human.
Research topic
Cardiovascular disease, Energy metabolism and body weight regulation, Lipoprotein metabolism
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Heparin
Sample Requirements
50 µl/well (Diluted to 100X with buffer solution ©)
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
2.5–160 ng/ml
Limit of Detection
0.198 ng/ml
Intra-assay (Within-Run)
n = 5; CV = 3.5%
Inter-assay (Run-to-Run)
n = 3; CV = 5.7%
Spiking Recovery
99.9 %
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